br Mathematical models and numerical methods br

Mathematical models and numerical methods

Numerical validation

Results and discussion
To investigate the effect of ultrasound wave on the flow structure and behaviors of RBCs, Fig. 9 shows the scheme of microvessel with ultrasound source with area of 225×60 lattice nodes (45μm×12μm). The periodic boundary condition is applied and SBI-0206965 flow moves directly from left boundary to right one. The ultrasound source (D=50 lattice nodes) locates at the top boundary of the microvessel. A region of interest (ROI) is selected, which fits a square of 15×15 lattice nodes for visualization of vector measurement. To investigate the effect of the ultrasound on the RBC behaviors, three different intensity of ultrasound cases are elected (I=0, 0.2 and 0.3). In addition, the detail plasma and RBC parameters employed in this section are listed in Table 1 from Refs. [18,31].

An immersed boundary lattice Boltzmann method considering ultrasonic effect is proposed to simulate red blood cell (RBC) aggregation and deformation in ultrasonic field. Numerical examples involving the lid driven flow and typical RBC behaviors in shear flow are presented to verify the method. In the following, the typical streamline, normalized out-of-plane vorticity contours and vector fields in pure plasma under three different ultrasound intensities are presented. Meanwhile, the corresponding transient aggregation behavior of RBCs, with special emphasis on the detailed process of the RBC deformation, is shown. The normalized vorticity profiles with/without RBC are also investigated, respectively. The primary findings include:
Regarding future directions, additional research through IB-LBM considering the thermal effect is needed to further advance the understanding of the effect of temperature on the dynamics of RBCs aggregation, as well as shape deformation. Additional numerical studies used by IB-LBM are also needed to improve the understanding of the interplay between inflow and cantilever beam, particularly for cases with spatial/temporally varying inflow, and for cases with rigid and/or elastic body motion [43,46]. Such research is important because accurate prediction of fluid-structure interaction is critical when analyzing the noise, vibration, and hydroelastic stability of hydraulic machineries.

The authors gratefully acknowledge the support by the National Natural Science Foundation of China (Grant Nos.: 51679005, 51306020 and 51479002), and the Open Foundation of State Key Laboratory of Hydraulics and Mountain River Engineering (Sichuan University, China). Additionally, the assistance of Yuanqing Xu in simulating the red blood cell is gratefully acknowledged.

From ancient times, people were attracted to perfumes and other fragrant materials. Nowadays, there is a growing need for high-quality textiles and packaging materials with antimicrobial properties for food safety, hygienic clothing, active wear, and wound healing [1]. Therefore, properly designed edible fragrant antimicrobial coating on textiles and packaging materials, would provide a significant contribution to the food, textile, medical, cleaning and toiletries industries. Several pathogenic bacteria and fungi were chosen for the antimicrobial testing due to their abundance as a community contaminator.
Ultrasound radiation is an excellent technique for the formation and adherence of organic [2,3] or inorganic [1] nanoparticles (NPs) to a large variety of substrates and for the deposition of NPs on flat and curved surfaces of ceramic [4–8], polymers [9,10], metals [11], and paper.
The edible fragrant solid flavors, Vanillin, RK, and camphor, inheritably possess antimicrobial properties, which have been known and used for centuries. Vanillin (4-hydroxy-3-ethoxybenzaldehyde), a pleasant smelling aromatic compound, is the world’s most popular flavor and fragrance compound [12,13]. It is widely used as flavoring agent in foods, beverages, pharmaceuticals, perfumes and cleaning products [14,15]. Vanillin is slowly oxidized on exposure to air and is slightly water soluble (10g/L) [12,16]. It displays antioxidant property [17,18] and gives antibacterial and antifungal activity against pathogenic microorganisms [12,18,19].

Most studies have shown successful USMB guided

Most studies have shown successful USMB guided drug delivery using already available clinical ultrasound imaging systems; however, the reported delivery efficiency is inconsistent [11], likely due to there being so far no standardization of the acoustic parameters. Although there are certain studies that have investigated microbubble parameters, including MB concentration, sound intensity, irradiation time, fundamental transmission frequency, duty cycle [19–23], most of these studies did not investigate the different combinations of various levels of parameters. What’s more, most of these studies investigate only one cell line and did not compare the sonoporation between tumor cell lines derived from different tissue.
Therefore, in this study, four kinds of human tumor cell line derived from different tissue including breast tumor (MCF-7), liver tumor (Bel7402), ovarian tumor (A2780) and thyroid tumor (ARO) were selected to reveal the effect of different tumor cell lines on sonoporation efficiency, and meanwhile use the orthogonal experimental design method to determine the impact of therapeutic parameters on sonoporation and determine the optimum parameter combination for each tumor cell line. To count the SBI-0206965 that are sonoporated, fluorescent dye (FD500) was used and counted by cytometry to evaluate transference percentage. It has been shown that sonoporated cells might undergo apoptosis, have poor cloning efficiency or can suffer from malfunction [24]. Therefore, safety remains an important factor to be addressed, and the cell survival rate was calculated with MTT test.

Materials and methods


Ultrasound-guided microbubble destruction has great potential for clinical translation in oncology because it is a safe, non-invasive, cost-effective and non-ionizing modality [29]. Importantly, this approach can create temporary and reversible openings in vessel walls and cellular membranes through a process called “sonoporation”, allowing enhanced transport of therapeutic agents across these biological barriers in the insonated region [10,30]. A number of molecules have transferred into cells with sonoporation including small fluorescent molecules, RNA, plasmid DNA, plasmid lipoplexes, nanoparticles, anti-cancer drugs, anti-bodies and viruses [7,31,32]. SonoVue is a suspension of stabilized sulfur hexafluoride (SF6) microbubbles which is isotonic to human plasma and very stable and resistant to pressure [33], and it is a commonly used microbubble to investigate sonoporation.
Most studies have shown successful USMB guided drug delivery using already available clinical ultrasound systems; however, the reported delivery efficiency is inconsistent [11], this phenomenon may have a variety of reasons, one of those may likely due to that the sonoporation maybe influenced by tumor cell lines from different tissue, and there being little research for this. It is also expected that optimized treatment schedules may need to be assessed for different tumor types to enable maximum treatment effects. Pichardo et al. has reported that various cell lines from the same tumor tissue (cervical-carcinoma-derived cells) impact the sonoporation [34]. Lamanauskas et al. has reported that human glioblastoma astrocytoma (U-87 MG) and colon cancer (HCT-116) cell lines showed different sonoporation efficiency of bleomycin under USMB treatment [28].
In this study, in order to assess whether the sonoporation efficiency is affected by tumor cell lines derived from different tissue, we selected four kinds of human tumor cell lines including breast tumor (MCF-7), liver tumor (Bel7402), ovarian tumor (A2780) and thyroid tumor (ARO). FD500 uptake assay was performed to assess cell membrane permeability changes. In addition, an orthogonal array experimental design based on three levels L9 (33) of three parameters (MB concentration, sound intensity and irradiation time) was employed to optimize the sonoporation efficiency for each tumor cell line. Finally, cell survival rate is also an important concern when carrying out the sonoporation experiment, therefore, MTT experiment for each group was also carried out.

Binding of purified P TSP to S Typhimurium O antigen

Binding of purified P22 TSP to S. Typhimurium O antigen is weak (Israel et al., 1972), and that of SP6 gp46 is presumably comparable. However, binding of P22 phage to O antigen is irreversible, implying significant cooperativity in binding of multiple TSPs, especially since hydrolysis of O antigen is required for P22 infection (Berget and Poteete, 1980; Iwashita and Kanegasaki, 1973; Schwarz and Berget, 1989). One difference between SP6 and P22 infection is that the P22 TSPs are not known to change orientation during infection. We thus suggest that the initial binding (and/or O antigen hydrolysis) of one SP6 gp46 TSP may only provide energy to disrupt the hand-over-hand garland. However, this step would facilitate binding of other TSPs, ultimately leading to rotation of the entire V-shaped TSP complex. We suggest that a similar process will describe not only SP6 gp47 recognition of the S. Newport O antigen but also K1-5 particles, whose two sets of TSPs differentiate between K1 and K5 capsulated cells (Scholl et al., 2001). There is no experimental information for these two phages.
This process is strikingly different from the mechanism used by T7 or T4 (Hu et al., 2013, 2015a). Binding of a single T7 or T4 long tail fiber to the cell surface has been suggested only to prevent the virion from diffusing away from its target cell, thereby providing time for other fibers to stochastically and non cooperatively dissociate from their SBI-0206965 on the phage tail and/or capsid and interact independently with their receptor.
The major tail proteins of SP6, gp32 and 33, are clearly homologous to those of T7, but an important recent in-depth analysis has revealed that they are homologous to the tails of essentially all podoviruses other than those in the Picovirinae subfamily (Hardies et al., 2016). Because SP6 grows on rough S. Typhimurium strains and the tail appears to make direct contact with the cell surface, whereas the TSPs do not (Fig. 6), tail proteins must be the major determinants of host specificity. The role of TSPs or tail fibers is then to facilitate a productive interaction of the tail with its receptor. By analogy with T7-like phages infecting Yersinia pestis, this receptor is likely the conserved lipid A-KDO region of the LPS (Kiljunen et al., 2011; Zhao et al., 2013). Supporting this conclusion is that a mutant of SP6: SP6coli, which contains four missense mutations in the tail genes 32 and 33, but no alteration in TSP genes, grows well in liquid cultures of laboratory strains of E. coli (Nguyen et al., 2012)(IJM unpublished); these strains have a different truncated LPS outer core than S. Typhimurium but have a comparable inner core.

Material and methods

This work was supported by grants R01GM110243 from the NIGMS (to JL and IJM), and AU-1714 from the Welch Foundation (to JL). KH is supported by grant R01GM056141 from the NIH. We are very grateful to Dean Scholl, who both provided S. Newport, S. Heidelberg, and SP6 mutants for this study and also communicated to us prior to publication that S. Newport is a host for SP6.

Epstein Barr virus (EBV) is a gammaherpesvirus that is highly proficient in establishing immunoevasive latent infections. Upon infection of B cells, the EBV genome is circularized as a minichromosome and chromatinized by host proteins to establish a restricted latent gene expression program in which only a small percentage of viral genes are expressed. During latency, the virus is subject to low-level spontaneous reactivation (Phan et al., 2016); however, in order to generate a productive lytic infection, the virus must overcome tight regulation to initiate complete reactivation. During latency, the EBV genome is completely chromatinized and subject to suppressive chromatin marks and DNA methylation (Hammerschmidt, 2015; Tempera et al., 2010). Significant efforts have explored changes in the chromatin landscape during reactivation, although it appears these changes are often cell line-dependent and likely consequential, not causal, to reactivation (Flower et al., 2011; Ramasubramanyan et al., 2012; Murata and Tsurumi, 2013). The molecular mechanisms driving the process of reactivation are understood primarily in the context of transcriptional regulation of the BZLF1 promoter (Zp). Numerous factors that bind to Zp have been identified as either positive or negative regulators of BZLF1 transcription, as well as a few cellular factors that contribute to lytic reactivation (McKenzie and El-Guindy, 2015). Understanding the molecular mechanisms dictating the latent to lytic switch may offer therapeutic insight for oncolytic therapy in latency-associated tumors. Further, if these mechanisms are conserved SBI-0206965 among herpesviruses, we could identify therapeutic targets to prevent lytic outbreaks in infected individuals.

TLC profile of UFW and ROFW when water extract was

TLC profile of UFW and ROFW when water extract was phase separated by ethyl…
Download high-res image (487KB)Download full-size image
Fig. 3. TLC profile of UFW and ROFW when water extract was phase separated by ethyl acetate, dried and dissolved in methanol recorded under short wave UV (A) and long wave UV (B) [GA: gallic acid; PCA: protocatecheuic acid; HBA: 4-hydroxybenzoic acid; HMBA: 4-hydroxy-3-methoxybenzoic acid; CA: caffeic acid; FA: ferulic acid; TCA: trans-cinnamic acid; SU: unknown SBI-0206965 in short wave UV; LU: unknown compound in long wave UV].
Download high-res image (493KB)Download full-size image
SSF is a complex biochemical process where several hydrolyzing enzymes like α-amylase, xylanase, β-glucosidase, esterases, etc., are produced, which are predicted to be associated in the release of water soluble and more bioavailable PC from insoluble bound form [4]. Along with enzymatic release of PC, some other unknown biochemical pathways might be involved in SSF process to increase the TPC and antioxidant properties of wheat. Moreover, in addition to PC, some other water soluble bioactive compounds like small peptides, xylo-oligosaccharides etc., produced during SSF might be contributing in the enhancement of antioxidant properties of fermented wheat [19,9,28].
4. Conclusion
Present study demonstrates that SSF of wheat by R. oryzae RCK2012 is a very fruitful method for the enhancement of TPC and antioxidant potential. On the basis of the results obtained, it is clearly indicated that water extract of ROFW has strong antioxidant property against several in vitro and an in vivo oxidative system compared to unfermented wheat. Predominantly insoluble bound phenolics in cereals are less bioavailable. To maximize the possible health benefits of cereals, SSF is a great option for the improvement of bioavailability of cereal phenolics by increasing their solubility. In comparison to unfermented wheat, consumption of fermented wheat might give more health protection against oxidative damages. Moreover, fermented extract can be served as powerful sources of natural antioxidants over the synthetic antioxidant compounds used very often in food and pharmaceutical industry. Additionally, along with PCs some other bioactive compounds might be produced during SSF, which were contributing antioxidant property. SSF process could be an innovative technology in cereal science research to develop nutrition rich and more healthy cereal products. Fermented wheat extract contains a complex mixture of phenolics. Further study is necessary to identify the unknown phenolic compounds.
The authors gratefully acknowledge the University Grant Commission, Govt. of India, New Delhi (No. F. 15-83/2011(SA-II))for financial assistance.
1. Introduction
Methanotrophic bacteria utilize CH4 as their sole carbon and energy source, and thus are important in the global carbon cycle [25]. They are highly diverse and found in a wide range of environments [9,25]. Most of the known methanotrophic bacteria belong to the Alphaproteobacteria and Gammaproteobacteria, and some Verrucomicrobia isolates are known to be methanotrophs [25]. They transform CH4 to CO2, with methanol, formaldehyde and formate as intermediates [9]. In the field of biotechnology, methanotrophs are a valuable biological resource because they can degrade the greenhouse gas methane, and co-metabolize various organic compounds [25,27]. Therefore, methanotrophs are used in environmental engineering systems to mitigate methane emission and to remove recalcitrant contaminants (e.g., trichloroethylene) [7,20,23].

Fig nbsp xA Non local cost aggregation for pixel p

Fig. 7. Non-local cost aggregation for pixel p on the cross-trees structure with a prior. Green arrows represent cost aggregation flow on horizontal scanlines and red arrows represent cost aggregation flow on the vertical scanlines. A cross-based structure which consists of these color arrows is the support region of pixel p. (For interpretation of the references to color in this SBI-0206965 figure caption, the reader is referred to the web version of this article.)Figure optionsDownload full-size imageDownload high-quality image (117 K)Download as PowerPoint slide
To a certain extent, our work is also motivated by the cross-based structures methods [19] and [20]. Instead of estimating the cross-based structures locally, we use the priors to locate potential local support regions? boundaries and then estimate the support regions as cross-structures on the horizontal and the vertical scanlines implicitly by cutting the cost aggregation flow as shown in Fig. 7.
Note chorion for the superpixel prior, there are over-segment boundaries even between neighboring pixels within low-textured regions. Such over-segment boundaries will not terminate the cost aggregations from further pixels on the path since the edge weights (equal to pixel similarity as defined in (7)) are still low.

Radiocarbon data of the Middle Holocene

Radiocarbon data of the Middle Holocene is derived from different site types: open-air sites (all of them shell middens; n = 5), with high and low densities, and rock shelters (n = 6, but from 2 sites). Evidence of exploitation of marine resources was recorded in shell middens ( Zubimendi et?al., 2005, Castro et?al., 2007 and Castro et?al., 2011), and also in Alero El Oriental. In this SBI-0206965 last case, mollusc shells and bones of pinnipeds were identified in low densities (Ambrústolo et al., 2011). This would suggest that during the Middle Holocene, the coast and its resources (mainly molluscs) have been exploited by hunter–gatherers who occupied the area.
There is no evidence of previous human occupation to smooth muscle time, although according to various studies, during the Pleistocene–Holocene transition and prior to the mid-Holocene transgressive maximum at ca. 8000 cal BP, the Atlantic coast would have been several kilometres eastward from its current position (Codignotto et?al., 1992, Schellmann and Radtke, 2010 and Ponce et?al., 2011). Therefore, the evidence of coastal occupations could be under the sea today, or have been destroyed by erosion of the advancing sea (Bailey and Milner, 2002 and Favier Dubois, 2013).