br Material and methods br Acknowledgement We thank Jane

Material and methods
Acknowledgement We thank Jane Hagelskjær Knudsen, Christian Knudsen, and Bettina Hansen for excellent technical assistance. This project was supported by Aarhus University and partially by the Lundbeck Foundation (R151-2013-14439) and Danish Council for Independent Research (DFF-1335-00763).
Resource details The study was approved by the ethics committee of 1st and 2nd Hospital of Jilin University, written informed consent was obtained from the patient. 1ml of peripheral blood sample was extracted from a 79-year-old sporadic male Parkinson\'s disease patient. The molecular mechanism of common sporadic (non-familial) PD is still unknown (Kalia and Lang, 2015; Peeraully and Tan, 2012). The PD-BP001 iPSC lines were derived using the CytoTune®-iPS 2.0 Reprogramming System (Thermo Fisher Scientific), including four Yamanaka factors Klf-4, c-Myc, Oct-4 and Sox-2 (Ban et al., 2011). The derived hiPSC lines displayed a typical ES cell morphology, a high nucleus/cytoplasm ratio and prominent nucleoli. The identity of derived iPSC lines was confirmed by immunofluorescence staining. After 7–9 passages, the absence of exogenous reprogramming transgenes was checked by RT-PCR. The differentiation capacity of hiPSC lines into three germ layers was investigated by in vivo teratoma formation assay. The derived hiPSC lines showed normal karyotype (46, XY).
Materials and methods
Author disclosure statement
Acknowledgments This study was supported by fund from National Medical Research Council (NMRC) of China to SZ (fund number: 30400447) and HW (fund number: 30500548).
Resource table. Resource details We generated a cell line (H9Nrf2KO-A13) that is a homozygous knockout of Nrf2 by targeting exon 4 of Nrf2 using the CRISPR/Cas9 gene editing system (Fig. 1A). H9 AN-2728 were electroporated with preassembled Cas9 protein/single-chain guide RNA (sgRNA) ribonucleoproteins (RNPs) targeting the transactivation domain of Nrf2 (Fig. 1B). Four days after electroporation, cells were reseeded as single cells to obtain single-cell-derived clones. To validate the Nrf2 knockout, we performed targeted deep sequencing. Among 106 clones, we found three homozygous mutant lines and a biallelic 14-nucleotide-deleted and 2-nucleotide-inserted line, H9Nrf2KO-A13 (Fig. 1C); these were expanded and stored for further characterization. The H9Nrf2KO-A13 line was characterized both in terms of its molecular phenotype and differentiation potential. The H9Nrf2KO-A13 line is morphologically normal (Fig. 1D). ICC data revealed that the H9Nrf2KO-A13 line is OCT4-, SSEA4-, TRA-1-60-, and TRA-1-80-positive (Fig. 1E). We confirmed that the CRISPR/Cas9 gene editing process did not introduce any chromosomal abnormality by determining the cell line\'s karyotype (Fig. 1F). To confirm its differentiation potential, we subcutaneously injected the clone into immunodeficient mice to test for teratoma formation that resulted in cell types representing the three germ layers (Fig. 1G). Additionally, the mRNA expression levels of markers representing the three germ layers were examined in 14-day-cultured embryonic bodies (EBs) (Table 1). Finally, to confirm the safety of the cell line, we tested for mycoplasma (data not shown), viruses, and bacterial infections (Fig. S1).
Materials and methods
Conflict of interest
Acknowledgements This work was supported by the Korea National Institute of Health (2016-NG61004-00).
Resource table. Resource details The study was approved by the ethics committee of 1st and 2nd Hospital of Jilin University, written informed consent was obtained from the patient. 1ml of peripheral blood sample was extracted from a 64-year-old male multiple schwannoma (MS) patient. This patient does not harbour SMARCB1 and NF2 mutations, which was often observed in familial MS cases (Jacoby et al., 1997; Smith et al., 2012). The MS-BP003 iPSC lines were derived using the CytoTune®-iPS 2.0 Reprogramming System (Thermo Fisher Scientific), including four Yamanaka factors Klf-4, c-Myc, Oct-4 and Sox-2 (Ban et al., 2011). The derived hiPSC lines displayed a typical ES cell morphology, a high nucleus/cytoplasm ratio and prominent nucleoli. The identity of derived iPSC lines was confirmed by immunofluorescence staining. After 7–9 passages, the absence of exogenous reprogramming transgenes was checked by RT-PCR. The differentiation capacity of hiPSC lines into three germ layers was investigated by in vivo teratoma formation assay. The derived hiPSC lines showed normal karyotype (46, XY).