In the present study Plackett

In the present study, Plackett–Burman experimental design was applied to screen the suitable operational parameters followed by RSM based on Box–Behnken design matrix for the optimization of biosorption process using immobilized bacterial ZJ 43 viz. Pseudomonas alcaligenes RJB-B and Pseudomonas resinovorans RJB-3 wherein the interactive effect of the most significant operating variables: pH, initial As(V) concentration and mixing speed were evaluated for removal of As(V) from aqueous solution. Moreover, the kinetics and mechanism of As(V) adsorption based on FTIR (Fourier transform infrared spectroscopy) and EDX (Energy-dispersive X-ray spectroscopy) analysis were also investigated. Although there are several reports on optimization of process parameters based on Plackett–Burman and RSM, but to best of our knowledge application of Plackett–Burman along with RSM based on Box–Behnken design for optimization of arsenate removal from aqueous system using immobilized bacterial biomass is not yet reported.